20 - 24 January, San Diego, CA
Iskandar Dib, VALIDOGEN's Head of Analytics & DSP and Roland Weis, VALIDOGEN's Head of Operations will be attending PEPTALK 2020.
Don't miss Iskandar's presentation:Boosting VHH Expression Using UNLOCK PICHIA
Wednesday, 22 January, 9.20 am - 9.50 am, "Effective Expression and Production of Recombinant Proteins" stream
VHHs, single-domain antibodies derived from camelid species represent the third generation of antibodies and are currently developed for an increasing number of different applications.
Efficient production of VHHs is enabled by VALIDOGEN’s yield-enhancing protein production toolbox known as UNLOCK PICHIA comprising a broad diversity of molecular tools and expression strategies for Pichia. We demonstrate significant enhancement of VHH expression by a set of helper factors acting along the way from transcription to secretion.
VHHs, derived from camelid species, the so-called “third generation” of antibodies and also known as sdAbs or Nanobodies®, feature a number of intriguing characteristics that make them superior for many applications. Compared to mAbs and antibody fragments (Fab and scFv), VHHs exhibit equally high specificities and affinities in antigen binding and show further advantages due to their small size, high solubility, thermal stability, design flexibility & modularity, good tissue penetration in vivo, ease of genetic engineering and expression in microbial systems.
As a consequence, VHHs are currently developed for an increasing number of different applications ranging from medical use as APIs (first approval of a Nanobody® - Caplacizumab - in 2018) and in vivo imaging reagents to diagnostic and analytical applications for diverse industries.
Dr. Roland Weis, VALIDOGEN’s Head of Operations, will contribute to the ‘Recombinant protein production processes at industrial scale’ session of the upcoming RPP10 conference on Day 3 (11.20 - 11.40 a.m., April 26) with his presentation on ‘Productivity through diversity - a protein production toolbox to UNLOCK PICHIA’.
“Novel product classes and current trends in biopharma production ask for versatile and yet robust expression systems. Our answer is a yield-enhancing protein production toolbox, ‘UNLOCK PICHIA’, that enables fine-tuning of protein expression by its diversity of molecular tools and expression strategies for Pichia,” Roland Weis explained.
VALIDOGEN to co-present with Boehringer Ingelheim at BPI International US West 2019, 11 - 14 March, Santa Clara, CA
Iskandar Dib, Principal Scientist Process Development & Analytics, VALIDOGEN GmbH
Stefan Krahulec, Senior Manager Technology & Innovation, Boehringer Ingelheim RCV GmbH & Co KG
"Best practice model for collaborative end to end process development and manufacturing using P. pastoris"
Monday, 11 March, 3:30 - 4:00 pm
BPI's Pre-Conference Symposium on Microbial Manufacturing
This joint talk will illustrate how the versatile genetic toolbox UNLOCK PICHIA, VALIDOGEN’s established strain generation strategies and seamless process transfer between the two companies enable fast track development from gene to GMP. In a case study example, Iskandar and Stefan will discuss strain and process improvements made, process development for Phase I and successful transfer to GMP scale.
Iskandar and Stefan will also participate in the "Thought Leaders Discussion: Where Is, Microbial Production Going?" on March 11, 9:30 - 10:00 am.
Topics to be discussed will cover the role of microbial hosts in the bioproduction space, technologies and product advances that will change the market for microbial production and drive industry away from traditional CHO platforms as well as the development of microbial platform approaches.
UNLOCK PICHIA - Broadest Pichia protein production toolbox
Learn more by exploring VALIDOGEN's Pichia pastoris protein production toolbox "UNLOCK PICHIA" or speak to one of our protein expression experts at one of the following events:
VTU Technology GmbH, ein führender Anbieter exklusiver Technologien und Services für die Entwicklung von Hochleistungsprozessen zur Proteinproduktion mit der Hefe Pichia pastoris, firmiert im Zuge einer Neuorganisation der VTU Holding GmbH ab sofort unter dem Namen VALIDOGEN GmbH.
In den 10 Jahren seines Bestehens, hat sich das Unternehmen erfolgreich als weltweit führender Dienstleister im Bereich der Pichia Prozessentwicklung etabliert.
You need stable, robust and scalable processes for commercial manufacturing?
Are you looking for a process yielding consistent product quality?
Are you seeking to acquire process knowledge?
Pichia processes at VTU show extraordinarily high space-time yields and typical product concentrations of 10 - 20 g/l at raw product purities of 80 - 90 % in culture supernatant before downstream processing.
Apply VTU's entire protein production toolbox to increase product quality and enhance product yields through comprehensive production strain development
Do you need to maximize your yield to be competitive?
Are you seeking to improve product quality of your protein?
Are you in search of high performance expression strains efficiently expressing your protein suitable for commercial protein production?
Straightforward determination of expression potential & rapid production of protein samples
You instantly need a few mgs of recombinant protein?
Expression in other systems is troublesome or failed?
Expression in commercially available yeasts was not satisfying?
Are you searching for a powerful expression system suitable for industrial protein production?
Competitive through diversity - boost protein yields & quality
Building on Pichia´s inherent potential, VTU established an exclusive yield-enhancing protein production toolbox, standing out due to its diversity of expression tools and expression strategies. We fine-tune the expression of your protein by unlocking and boosting Pichia´s potential for effective production of fully active, high quality proteins.
Discover the elements of VTU´s toolbox